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Applications of Flow Cytometry in Stem Cell Research and by Awtar Krishan,H. Krishnamurthy,Satish Totey

By Awtar Krishan,H. Krishnamurthy,Satish Totey

A much-needed primer at the use of laser stream cytometry for stem phone analysis

Laser circulate cytometry is a strong instrument for quick research of cells for marker expression, telephone cycle place, proliferation, and apoptosis. in spite of the fact that, no assets particularly tackle using this technique for the research of stem cells; this is often specially vital as stem telephone research comprises really expert equipment and marking techniques in response to particular features reminiscent of marker expression, mobile dimension, drug shipping, and efflux of the stem cells.

Now, this booklet reports those methods, discusses the technology in the back of them, and offers real-world examples to demonstrate the usefulness of the tools. It brings jointly world-class specialists in pathology, biophysics, immunology, and stem telephone study, who draw upon their broad adventure with the tools and convey examples of excellent facts to assist consultant researchers within the correct direction.

Chapter assurance includes:

  • Stem telephone research and sorting utilizing part population
  • Flow cytometry within the examine of proliferation and apoptosis
  • Stem mobile biology and application
  • Identification and isolation of very small embryonic-like stem cells from murine and human specimens
  • Hematopoietic stem cells—issues in enumeration
  • Human embryonic stem cells: long term tradition and cardiovascular differentiation
  • Limbal stem cells and corneal regeneration
  • Flow cytometric sorting of spermatogonial stem cells
  • Breast melanoma stem cells
  • Stem mobile marker expression in cells from physique hollow space fluids

This ebook is a vital source for all graduate scholars, practitioners in constructing international locations, libraries and publication repositories of universities and study associations, and person researchers. it's also of curiosity to laboratories engaged in stem cellphone learn and use of stem cells for tissue regeneration, and to any association dealing in stem telephone and tissue regeneration research.

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